How close may beads be to the image edge or to one another before they are rejected?
Huygens Pro can average a number of beads and is able to reject beads too close to the edge or to another microsphere -but how close?
Around 30 optical units — an optical unit is (for NA=1.3 and lambda = 500nm) 61nm — so about 2 micron. Because the software doesn't know whether there is a bead just over the edge of the image, the bead-edge rejection distance is the same.
Keywords: averaging beads edge optical unit
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux
Related products: Hu Pro
