FP6 AUTOMATION project

Three-dimensional (3D) fluorescence imaging microscopy of individual living cells is an essential tool for cellular biology, pathology and the study of infection and virulence therein. However, today, one critical constraint of established techniques is that samples must be stabilised by attachment to an optically transparent surface, thereby completely precluding their use for non-adherent cell types.

The severity of this limitation becomes clear when one considers that for basic- and biomedical-research, cell-based assay and cell-diagnostic applications some of the most important targets are non-adherent cells, for example, stem cells, systemic cancer cells and lymphocytes. Our consortium therefore propose a completely new 3D imaging strategy targeted specifically at live, non-adherent cells.

See the Automation Project web page.

For details on SVI participation, see Automation Project Details (private article).

Work in progress

• Micro Rotation Workbench (MRW aka MRS)
• Automation Cage Stability
• Automation Two Views

---