How to analyze images?
How to use the 'Analyze labeled image' tool that can be found in Huygens Professional?
Deconvolve first:
Thresholding noisy images is tricky since voxels from noise can be seen as objects. It's a good idea first to deconvolve your image and then find a threshold which excludes all background.
Proceed with the analysis:
- Directly label a source image using labelDirect. Syntax:
source labelDirect -> <destination> <threshold> <seedThreshold> ?-frame <frame>? ?-chan <channel>? ?-garb <volume>?
- Do the actual analyzing: Select the ORIGINAL image and use 'Analyze labeled image' to get information about the numbered objects.
It calculates the total volume (number of voxels in the objects, the minimum and maximum value in the objects, the sum of all voxels in the objects and the location of their Center of Mass).yourImage labelDirect -> b 100 150 yourImage analyze b
Keywords: analysis threshold analyze labeled image
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Linux
Related products: Hu Pro Hu Script
