How should the acquisition zoom factor be taken into account in the calculation for the backprojected pinhole diameter?
The aquisition zoom factor only affects the scanning parameters and as far as we know has no effect on the computation of the backprojected pinhole size. <br>
If the pinhole is specified in terms of Airy units the backprojected radius is computed as (from the FAQ):
backprojected_RADIUS = number_of_Airy_disks * 0.61 * lambda / NA
To compute the backprojected radius from the physical pinhole size see the tables in the Huygens Essential or Professional User Guide.
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Keywords: Airy zoom factor pinhole size<br>
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs<br>
Platforms: Irix Linux Windows Mac AIX<br>
Related products: Hu Ess Hu Pro<br>
