Is deconvolution on 2D or 2D-time images possible?
Yes. The results are especially remarkable for Wide Field Microscopes.
A 2D image recorded with a microscope can be considered as a slice from a 3D image. In this case the Huygens Software treats the data as a (severely) truncated 3D image, but still 3D. When deconvolving it Huygens attempts to reconstruct blur sources outside the slice and remove blur from it, regardless of the time frames.
Proper parameters
When doing 2D deconvolution of widefield images, set the Z Sample Size to the ideal Nyquist value. You can calculate this by using the Nyquist Calculator.
If you have a 2D time series, make sure Huygens interprets the series as such. Some File Formats having indexes in the file name are interpreted as 3D stacks by default: you may need to convert the dataset once opened from XYZ to XYT. See Convert The Data Set. Failing in doing so will produce a wrong deconvolution, as explained in this other FAQ.
Keywords: 2D time "single slice"
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac AIX
Related products: Hu Ess Hu Pro
