Can a 2D-time series be deconvolved as 3D stack?
Huygens can convert a 2D-Time Series into a 3D stack. Would deconvolution of the resulting 3D image and then converting back to 2D-time yield the same result as doing a 2D-time deconvolution?
When you deconvolve a 2D-time image as being a 3D image, you force the software to wrongly assume that the relationships between the 2D slices, if any, are due to the axial imaging properties of the microscope. But obviously they are not!!! The results will be therefore incorrect.
Instead, when you correctly process the 2D-Time Series as such with the Time option the software will, after correcting for bleaching and variable background, properly deconvolve each 2D image as a 3D data set with one plane recorded and the rest missing, as explained here.
(Some File Formats having indexes in the file names are interpreted as 3D stacks by default: you may need to convert the dataset from XYZ to XYT once opened.
As an alternative you could write a Tcl script for Huygens Scripting or Huygens Professional to deconvolve the 2D-time series frame by frame. Still, your images would not be corrected for bleaching and varying background, a task automatically performed if you have the Time option.
In case of a 3D-time series, with the Time option your data will be also corrected for axial drift.
Keywords: 2D time bleaching axial drift
Categories: Faq Deconvolution, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac AIX
Related products: Hu Ess Hu Pro Hu Script
