How do I convert a collection of 2D files into a format that Huygens can deconvolve as a 3D stack?
Rename your successive planes (files) to make them a series with equal base names, like:
yourFile1.tiff -> newFile000.tif (or tiff) yourFile2.tiff -> newFile001.tif etc.
Huygens will read the stack as a single 3D image by selecting the image
newFile.000. (Or you may skip the first N planes by opening newFileN).In case you have multichannel images or time series you have to follow the Leica-style name convention in order to read in the tiff series as a single image. See Renaming Tiff Series and Leica Tiff.
Keywords: 2D conversion Leica style
Categories: Faq Files, Huygens Faq, Imported Faqs
Platforms: Linux Windows Mac
Related products: Hu Ess Hu Pro
